解:
$\begin{aligned}&(5x^{2}y - 2xy^{2}-3xy)-(2xy + 5x^{2}y - 2xy^{2})\\=&5x^{2}y - 2xy^{2}-3xy - 2xy - 5x^{2}y + 2xy^{2}\\=&(5x^{2}y - 5x^{2}y)+(-2xy^{2}+ 2xy^{2})+(-3xy - 2xy)\\=&-5xy\end{aligned}$
当$x = -\frac{1}{5},$$y = -\frac{1}{3}$时,原式$=-5×(-\frac{1}{5})×(-\frac{1}{3})=-\frac{1}{3}$